Background: The interplay of ethical stress, heavy workloads, and job dissatisfaction can hinder the recruitment and retention of healthcare professionals (HCPs). Person-Centred Care (PCC), rooted in ethical principles, involves collaborative care and is associated with the challenges. Prior research in the PCC@work project on the impact of PCC on HCPs' work-related health and job satisfaction has yielded mixed results 1. Our cross-sectional study found significant associations2 and our qualitative study review suggest improved job satisfaction and role changes3. Neither provide tangible solutions to recruitment and retention challenges. Understanding how the application of PCC influences HCPs across different settings requires further exploration through rigorous methodology. Aim: The overall aim of PCC@Work is to follow, describe and assess the impact of applying PCC in hospital, municipality and regional primary care on work-related health and job satisfaction among HCPs, and to explore HCPs' experiences of their work-related health and job satisfaction while applying PCC. Methods: The study design is a prospective, longitudinal cohort study combined with qualitative focus group study. A web-based questionnaire will be distributed at intervals to HCPs in hospital wards, municipality and regional primary care centres in The Västra Götaland Region, Sweden. In addition, focus groups will be conducted with HCPs from different healthcare settings. Discussion: Optimising HCPs’ work environment is vital to retain the workforce and safeguard the quality of care. PCC can form a critical component for effective change in the work environment of HCPs. PCC@Work will highlight some of the knowledge gaps on the effects of PCC on work conditions of HCPs by including diverse professions and underrepresented healthcare settings. The uniqueness of the PCC@work project lies in the all-encompassing longitudinal approach, blending quantitative and qualitative methods, involving various professions and settings, to explore HCPs' work-related health and job satisfaction under PCC.