Background: Worldwide, the healthcare sector faces challenges in retaining healthcare professionals, often driven by work-related health factors such as job dissatisfaction and stress of conscience. Person-centred care (PCC), rooted in ethical principles, promotes collaborative care co-created by HCPs and patients, may help address these issues, though its impact across professional groups needs further attention. This study aimed to determine the association between perceived PCC and the experience of stress of conscience among primary healthcare professionals and whether this association is mediated by job satisfaction and moderated by profession. Methods: A cross-sectional design with a moderated mediation analysis was conducted using baseline data from the longitudinal PCC@Work project. A questionnaire on PCC and work-related health was distributed among registered nurses, physicians, psychologists, midwives,  assistant nurses and other professions in western Sweden. Results: The results showed that among the 560 respondents, the different professions’ scores were relatively close, but modelling all concepts revealed variations in their relationships. Profession moderated these associations, slightly reducing the strength of the relationship between PCC and job satisfaction (b= 1.02 to 0.98), while nearly halving the overall strength of associations between PCC, job satisfaction, and stress of conscience (b = –1.44 to –0.79), indicating varying impacts across professions. Conclusion: Type of profession significantly moderated the association between PCC and stress of conscience and job satisfaction. This may point at a need for tailored implementation strategies to optimize PCC's impact in primary care. Various professional groups experienced PCC's effects differently and customized approaches may also be needed to support their work-related health effectively.